SNIC SUPR
Single-cell Transcriptomics of Developing Brainstem Respiratory Networks
Dnr:

SNIC 2019/8-111

Type:

SNIC Small Compute

Principal Investigator:

Wiktor Phillips

Affiliation:

Karolinska Institutet

Start Date:

2019-03-14

End Date:

2020-04-01

Primary Classification:

10612: Biological Systematics

Webpage:

Allocation

Abstract

From the moment we take our first breath, brainstem networks that drive rhythmic breathing behavior and ventilatory chemoreflexes must be sufficiently developed to support life. Specific nuclei in the brainstem medulla develop rapidly to meet such demand, but are still nascent and vulnerable to biological stress during early postnatal development. Developmental perturbations, such as being born preterm, or infection, can be particularly destabilizing in the infant and may result in cardiorespiratory failure. This project leverages single-cell transcriptomics as a means of defining the developmental trajectory of brain cells which produce breathing motor rhythms and respond to changes in the environment. By following the development of these cell populations through the perinatal stage of development and after inflammatory challenge, we aim to clarify the mechanisms in play when infants experience respiratory dysfunction and how it may be treated.