SNIC SUPR
The biology of pericytes
Dnr:

SNIC 2019/8-368

Type:

SNIC Small Compute

Principal Investigator:

Robert Carlsson

Affiliation:

Lunds universitet

Start Date:

2019-12-17

End Date:

2021-01-01

Primary Classification:

30105: Neurosciences

Webpage:

Allocation

Abstract

We wish to study the biology of pericytes in different pathologies by transcriptional analysis by scRNAseq were we want to extract FASTQ files from BCL2 reads in 10x cell ranger. We then want to align the reads to a reference genome with e.g. Kalisto. (This is mainly because our sequencing facility do not map to GFP in their sullied alignments , and we have GFP as a reporter for a specific cell type). Furthermore we would like to run a quality assessment of the data, preferably with 10x cell ranger. We will analyse both scRNAseq analysis and snucRNAseq data.