Chromosome-wide gene regulation in Drosophila
The only chromosome-wide targeting mechanisms recognised until recently were the dosage compensation mechanisms that equalize the transcriptional activity of the single male X-chromosome and the two female X-chromosomes. However, our discovery that POF is involved in global regulation of the genes of Drosophila’s chromosome 4 provided the first evidence of another chromosome-specific transcription mechanism apart from the sex-chromosome dosage compensation mechanisms. The current proposal includes the continued dissection of the two known chromosome-wide systems. We use ChIP-seq, RNA-seq and rip-seq techniques to address four main issues: (1) the evolution and function of chromosome-wide targeting mechanisms (2) The correct targeting of chromatin associated factors; (3) genome-wide fine-tuning of transcription output and (4) establishment and propagation of chromatin structure within and between chromosomes.