Oat seeds contain many health enhancing bioactive molecules. However, to develop oat as a functional food for the future, ß-glucans, protein and avenanthramide contents could be enhanced even further. To facilitate such a development, an increase in the variation of the breeding material would be an advantage. Recently we produced a mutagenized oat population of more 2,500 lines originating from the commercial variety SW Belinda, where more than one million mutations have been introduced into each line. Taking advantage of high precision biochemical selection methods, we then identified lines with more than 50% enhanced levels of ß-glucans, phytosterols, lipids, proteins, avenanthramides and sapponins in the population.
We will now use RNA-Seq to determine the specific mutations and molecular mechanisms behind the modified characters of the mutant lines. The first step will be the assembly of the Belinda developing seed transcriptome. Subsequent sequencing projects will utilise tissue collected from the mutants, where we will utilise differential expression analysis and amino acid mutation detection to explain each specific phenotype in selected mutant lines.