A typical eukaryotic genome harbours a tremendous variety of small regulatory RNAs (sRNA). The vast majority of sRNAs are processed from longer precursor transcripts. MicroRNAs (miRNAs) and transfer RNA fragments (tRFs) represent some of the most abundant classes of sRNAs. miRNAs and tRFs regulate gene expression by multiple processes. By interacting with specialised RNA processing proteins, they can serve as a guide and regulate the expression of protein-coding transcript or as a decoy and sequester away regulatory elements. This project is designed to interrogate the role of sRNA during liver regeneration in human. We are using small RNA sequencing (sRNAseq) technology to identify the sRNA population of normal and liver cells that have been undergone regeneration. Our long-term goal is to understand how sRNAs (and their interacting protein partners) facilitate cell proliferation and growth in regenerative processes.