Conformational change of TCR upon binding MHC 1/peptide

Dnr:

SNIC 2017/3-20

Type:

SNAC Small

Principal Investigator:

Renhua Sun

Affiliation:

Karolinska Institutet

Start Date:

2017-03-02

End Date:

2018-03-01

Primary Classification:

30108: Cell- och molekylärbiologi

Webpage:

http://ki.se/en/meds/research-group-adnane-achour

Allocation

Abstract

A necessary feature of the immune system, TCR (T-cell receptor) cross-reactivity has been implicated in numerous autoimmune pathologies and is an underlying cause of transplant rejection. Early studies of the interactions of αβ TCRs (T-cell receptors) with their peptide–MHC ligands suggested that conformational plasticity in the TCR CDR (complementarity determining region) loops is a dominant contributor to T-cell cross-reactivity. Since these initial studies, the database of TCRs whose structures have been solved both bound and free is now large enough to permit general conclusions to be drawn about the extent of TCR plasticity and the types and locations of motion that occur. In the present paper, we review the conformational differences between free and bound TCRs, quantifying the structural changes that occur and discussing their possible roles in specificity and cross-reactivity.Here We describe a computational approach to the study of the immune synapse. Using molecular dynamics simulation, we studied the smallest viable model, a MHC 1/peptide complex, to reveal conformational change of TCR upon binding MHC 1/peptide.