In silico mutational analysis of Hepatitis C drugs and analysis of inhibitors to Zika virus

Dnr:

SNIC 2017/1-213

Type:

SNAC Medium

Principal Investigator:

Johan Lennerstrand

Affiliation:

Uppsala universitet

Start Date:

2017-04-28

End Date:

2018-05-01

Primary Classification:

30209: Infektionsmedicin

Webpage:

Allocation

Abstract

Dasabuvir is an approved drug against HCV that inhibit the viral NS5B RNA-polymerase. In a previous study, we sequenced patients samples from Sweden using next generation deep sequencing (PacBio). This resulted in the identification of single nucleotide polimorphisms associated with resistance to dasabuvir. We are now intersted in simulating how these mutations can affect the binding affinity of dasabuvir to the HCV RNA-polymerase. To do so will use GROMACS to perform molecular Dynamics simulation of WT and mutated versions of NS5B in complex with Dasabuvir. We will also use the allocated resources to perform more simulations of ZIKV protease in complex with potential inhibitors.