Macrophages are crucial cell types that eliminate foreign cellular invaders (such as cell debris, toxic substances, microbes or cancer cells) via a process called phagocytosis. In the liver, these macrophages are called Kupffer cells, which not only break down substances such as red blood cells to recycle iron from the heme but also alcohol-induced endotoxins. A cascade of signalling events facilitates proper macrophage function. This project is designed to interrogate the role of small regulatory RNAs (sRNAs) in mice subjected to normal and high-fat diet. We are using small RNA sequencing (sRNAseq) technology to identify the sRNA population in macrophages present before and after high-fat diet. Our long-term goal is to understand how sRNAs (and their interacting protein partners) facilitate phagocytotic roles upon altered nutrient conditions.