Background: mesenchymal stem cell (MSCs) transplanted following spinal cord injury (SCI) can reduce neuroinflammation, spare tissue and enhance recovery of hind limb motor function. However, the mechanism of action of MSCs is not fully understood.
Aim: we aim to describe the mechanism of action of MSCs in vivo. Additionally we aim to study the effect of transplanted MSCs on immune cells of the central nervous system.
Material and methods: MSCs will be transplanted into the SCI area in the acute phase of the SCI. During the sub acute phase MSCs and immune cells will be isolated. RNA from isolated cells will be isolated and sequenced. Differential gene expression will be conducted using appropriate controls.