The project consists of several parts:
Sequencing of Wolbachia genomes from several different species of Drosophila using whole genome amplified DNA sequenced with both Illumina and PacBio. The aim is to identify genetic components involved in the phenotypic expression of Wolbachia in Drosophila.
Sequencing total DNA of Drosophila with Illumina, mainly D. paulistorum for investigation of mitochondrial diversity and semispecies specific genes and diversity.
RNAseq from D.paulistorum to look at the effect of Wolbachia on gene expression and investigate allele specific expression in hybrids.