SNIC
SUPR
SNIC SUPR
AMF_nuclei
Dnr:

SNIC 2017/1-527

Type:

SNAC Medium

Principal Investigator:

Anna Rosling

Affiliation:

Uppsala universitet

Start Date:

2017-11-24

End Date:

2018-12-01

Primary Classification:

10615: Evolutionary Biology

Secondary Classification:

10699: Other Biological Topics

Tertiary Classification:

10606: Microbiology (medical to be 30109 and agricultural to be 40302)

Allocation

Abstract

This is a project funded by ERC that will try to elucidate the genomic organisation within Arbuscular Mychorrhizal Fungi. We are sorting individual nuclei using the FACS machine in SciLifeLab. The nuclei are amplified using MDA whole genome amplification and sequenced in batches of 24 using Illumina HiSeqX. We will be repeating this procedure with multiple species, which will suppose a big amount of data stored. The genomes will be assembled and posterior phylogenomic analysis are expected using concatenated orthologs between species