SNIC
SUPR
SNIC SUPR
RNA-seq_1.688satellite
Dnr:

SNIC 2017/7-268

Type:

SNAC Small

Principal Investigator:

Jan Larsson

Affiliation:

UmeƄ universitet

Start Date:

2017-12-01

End Date:

2019-02-01

Primary Classification:

10609: Genetics (medical to be 30107 and agricultural to be 40402)

Webpage:

Allocation

Abstract

The aim of the current project is to test how genome-wide transcription is affected by removal of large (Mbp) blocks of repeated sequences. In particular we will focus on the 359 bp 1.688 satellite in D. melanogaster. Importantly, the 1.688 satellite is enriched 50-fold on the X-chromosome compared to the autosomes and it provides an exciting new unexplored link between our chromosome-specific targeting system mediated by POF and an ancient dosage compensation system. We will analyse genome-wide transcription from wildtype males and females and compare this to Pof mutants, to flies that lack ~3Mbp of 1.688 sequence and to a CRISPR generated mutant that lacks a small 1.688 repeat central on the X-chromosome.