SNIC
SUPR
SNIC SUPR
Zebrafish genome analysis
Dnr:

SNIC 2017/7-308

Type:

SNAC Small

Principal Investigator:

Hwei-yen Chen

Affiliation:

Uppsala universitet

Start Date:

2017-12-08

End Date:

2019-01-01

Primary Classification:

10609: Genetics (medical to be 30107 and agricultural to be 40402)

Allocation

Abstract

In this project, I propose to use the zebrafish (Danio rerio) as a model organism to study the connections among advanced paternal age, germline selection, and population genetic burden. The zebrafish is a particularly amenable model for studying germline genomics because its genome has been fully sequenced and extensively studied. Moreover, the zebrafish genome shows a striking homology to the human genome – approximately 70% of zebrafish genes have orthologous human genes. Besides, as most fish species, zebrafish reproduce via external fertilization, which not only facilitates easy in vitro sperm manipulation but also minimizes maternal influences in mutation-rate estimation – for example, in internally fertilized species, females may be choosy among mates and preferentially reproduce with males with a lower germline mutation load; in systems such as humans, where parental age at reproduction cannot be controlled, maternal age is often strongly correlated with paternal age, rendering it difficult to precisely estimate the age effect on mutation load.