Non-small cell lung cancers (NSCLC) are malignancies with a high mutational load.
The aim of this study was to evaluate the mutational status of patients included in a Swedish NSCLC cohort by targeted “next generation sequencing” (NGS). Target enrichment was performed on 354 patients from an unselected Swedish NSCLC cohort consisting of surgically resected specimens collected between 2006 and 2010. The gene panel (Haloplex, Agilent Technologies) covers all exons of 82 genes (total coverage 0.467kBp) and utilizes multiple amplicons/region and capture of both + and – strands. Sequencing data were analyzed with an in-house bioinformatics pipeline.