SNIC
SUPR
SNIC SUPR
The pre-mating social environment of zebrafish males affects gene expression during their offspring's early development
Dnr:

SNIC 2017/7-327

Type:

SNAC Small

Principal Investigator:

Willian Silva

Affiliation:

Uppsala universitet

Start Date:

2017-12-14

End Date:

2019-01-01

Primary Classification:

10615: Evolutionary Biology

Webpage:

Allocation

Abstract

The environmental conditions to which a population is exposed can be crucial to determine certain phenotypes. This has been repeatedly shown in studies of phenotypic plasticity as well as non-genetic inheritance, e.g. maternal effects. These conditions can affect populations at the organismal level as well as across generations. We manipulated the pre-mating social environment of zebrafish males and performed \textit{in vitro} fertilizations in a cross-and-split experimental design to look for signals of the parental environmental conditions on the transcriptome of the developing offspring. Additionally, we looked at sperm traits of the males under the different experimental treatments and simulated the possible consequences of changes in developmental traits in terms of population dynamics. We found that genes involved in translational control are differentially expressed in offspring from males under different stress-related social treatments. Social treatment also had a significant effect on sperm velocity after activation, indicating shallower curvature in sperm velocity in males under social stress. Our simulations predict the population effects of developmental delay, hatching and embryonic death rates, providing a potential mechanism for the effects of developmental traits on population dynamics.