SNIC
SUPR
SNIC SUPR
Dog_Reference_4
Dnr:

SNIC 2017/7-364

Type:

SNAC Small

Principal Investigator:

Gerli Rosengren Pielberg

Affiliation:

Uppsala universitet

Start Date:

2017-12-20

End Date:

2019-01-01

Primary Classification:

10609: Genetics (medical to be 30107 and agricultural to be 40402)

Webpage:

Allocation

Abstract

The project will aim for producing the next improved canine genome. The latest techniques in genome sequencing field will aid us to solve the currently problematic regions of the canine genome - the GC-rich regions, promoter regions, as well as regions with gene clusters presenting with high sequence similarities etc. We will produce 100x PacBio, Chromium, HiC proximity-guided scaffolding and RNA sequencing of 15-20 tissues, all from the same individual. Such data will enable us to produce a hybrid assembly, with improved resolution towards structural variants and currently unresolved genomic regions. Furthermore, additional dogs from other breeds will be added for the analysis of population structures. The produced enhanced genome assembly will help both researchers in our laboratory, as well as our international colleagues in resolving any canine genetic research question.