SNIC
SUPR
SNIC SUPR
Mycobacterium Genome and Transcriptome
Dnr:

SNIC 2017/7-415

Type:

SNAC Small

Principal Investigator:

Leif Kirsebom

Affiliation:

Uppsala universitet

Start Date:

2017-12-28

End Date:

2019-04-01

Primary Classification:

10606: Microbiology (medical to be 30109 and agricultural to be 40302)

Webpage:

Allocation

Abstract

Mycobacterium marinum (Mma) is a model system used to identify factors important for the establishment of tuberculosis that is caused by Mycobacterium tuberculosis (Mtb). We have sequenced several isolates of Mma from fish and human isolates. We now want to make a more systematic study where we will get access to a larger number of human and fish isolates which will enable us to study genomic differences between human and fish isolates in search for traits that lead to preferential infection of one or the other host species. We have also studied the transcriptomes of Mma under various growth and stress conditions and this will continue. We have further sequenced the genomes of most other mycobacteria (140 unique species; >200 genomes in total) and have generated transcriptome data for a selected number of these. This will lead to a more complete understanding of mycobacterial phylogeny and evolution and the development of pathogenic strains like Mtb. Our further analysis of existing data and continued generation of new genome and transcriptome sequencing data will require that we get access to the storage resources and computing power necessary for this kind of project.