The role of non-coding RNA during growth and development.
In this study we will investigate the role of small non-coding RNAs, e.g. miRNA, during growth and development. We will also try to understand the function of different proteins involved in gene silencing by small RNAs. Furthermore, this study will help us to understand how miRNAs recognize their targets and thereby regulate gene expression.
For these studies we use the well established and genetically tractable model organism Dictyostelium discoideum, a unicellular amoeba that upon starvation form multicellular structures.
We will isolate RNA from different strains/conditions (biological duplicates) and sequence small RNA and mRNA from the same RNA/cells.