SNIC
SUPR
SNIC SUPR
Function of RNAi and microRNA in Dictyostelium 2015 mRNA
Dnr:

SNIC 2017/7-401

Type:

SNAC Small

Principal Investigator:

Fredrik Söderbom

Affiliation:

Uppsala universitet

Start Date:

2017-12-29

End Date:

2019-01-01

Primary Classification:

10602: Biochemistry and Molecular Biology

Allocation

Abstract

The role of non-coding RNA during growth and development. In this study we will investigate the role of small non-coding RNAs, e.g. miRNA, during growth and development. We will also try to understand the function of different proteins involved in gene silencing by small RNAs. Furthermore, this study will help us to understand how miRNAs recognize their targets and thereby regulate gene expression. For these studies we use the well established and genetically tractable model organism Dictyostelium discoideum, a unicellular amoeba that upon starvation form multicellular structures. We will isolate RNA from different strains/conditions (biological duplicates) and sequence small RNA and mRNA from the same RNA/cells.