SNIC
SUPR
SNIC SUPR
Characterization of intestinal healing processes
Dnr:

SNIC 2017/7-445

Type:

SNAC Small

Principal Investigator:

Eduardo Villablanca

Affiliation:

Karolinska Institutet

Start Date:

2018-01-11

End Date:

2019-04-01

Primary Classification:

30110: Immunology in the medical area

Allocation

Abstract

Inflammatory bowel disease (IBD) is a chronic inflammatory condition targeting both the small and large intestine and affect patients worldwide, with high prevalence in Europe and in the US. Genome-wide association studies (GWAS) have been instrumental identifying novel genes, pathways and biological processes associated to IBD. However, a functional characterization of these genes, pathways and/or microbial relationships is still missing. This project aims to characterize intestinal immune functions involved in intestinal healing by using a time-series clustering approach. In particular, we will use RNA-seq, single cell sequencing (scRNA-seq), flow cytometry (FACS) and bacterial 16S sequencing analysis to identify the biological processes involved in epithelial cell function and inflammation regulation at steady-state and inflammatory conditions. Candidates genes and pathways will be later validated and/or studied in vivo.