SNIC
SUPR
SNIC SUPR
Transcriptome analysis of Chondrihthyan teeth and dermal odontodes
Dnr:

SNIC 2018/8-8

Type:

SNAC Small

Principal Investigator:

Tatjana Haitina

Affiliation:

Uppsala universitet

Start Date:

2018-01-15

End Date:

2019-02-01

Primary Classification:

10614: Developmental Biology

Webpage:

Allocation

Abstract

The chondrichthyan fishes have enigmatic hypermineralized tissue covering their teeth and dermal odontodes called enameloid. The molecular composition of enameloid matrix remains unknown, because the genes coding for secretory calcium phosphoproteins (SCPP) that are forming the matrix of hyperminerlized tissues on teeth of bony vertebrates are missing from the chondrichthyan genomes. We have dissected lower jaws and fin spines/scales from two chondrichthyan species, elephant shark, Callorhinchus milii and small spotted catshark, Scyliorhinus canicula. We have carried RNA-seq transcriptome sequencing at the SciLifeLab SNP&SEQ Technology Platform in Uppsala. The project will generate transcriptome data for several samples of fin spines, jaws and scales from different stages of development. This data will likely provide information about genes expressed during matrix deposition in the teeth and dermal odontodes of cartilaginous fishes. We will need UPPMAX CPU time to assemble transcriptomes using Trinity. The first transcriptome data has already arrived (delivery 00604). This project is supported by VR international postdoc grant 4.1-2016-00552.