SNIC
SUPR
SNIC SUPR
Assembly of the Picea abies mitochondrial genome
Dnr:

SNIC 2018/5-25

Type:

SNAC Small

Principal Investigator:

Alexis Sullivan

Affiliation:

UmeƄ universitet

Start Date:

2018-02-21

End Date:

2019-03-01

Primary Classification:

10607: Botany

Webpage:

Allocation

Abstract

One component of the total conifer genome, the DNA sequence of the mitochondrion, has yet to be robustly assembled. Our goal is to produce reference-quality genomes for six Norway spruce individuals originating from genetically and demographically distinct populations. To accomplish this goal, we have sequenced isolates enriched for mitochondrial DNA to to 20-50x on the PacBio system for two individuals, which will be used for de novo assembly. For the remaining four individuals, we have Illumina paired-end libraries from total genomic DNA, with an expected coverage of the mitochondrial genome of at least 50x.