Few phenomena have such strong climatic and long-lasting environments impacts as glaciations. But do we have a correct understanding about the interactions between plants and ice-sheets during glacial cycles? This project challenge our traditional view of ice-sheets being the final frontier for plants (and animals) and test a hypothesis stating that small terrestrial ecosystems developed on the European ice-sheet (Weichsel) millennia prior to deglaciation. The project use analyses of contemporary plant material along with ancient DNA (aDNA) preserved in glacial debris—being a largely unexplored archive of information regarding bioclimatic conditions during glacial cycles—to retrieve information about past supraglacial environments. More precisely we attempt to (1) analyze the genomic DNA ancient pine trees (Pinus sylverstris) to examine their origin and (2) examine ancient DNA from lake sediments and soils to reconstruct the historic ecosystems. Therefore we need resource at HPC2N for bioinformatics analysis.