SNIC
SUPR
SNIC SUPR
CIM-seq
Dnr:

SNIC 2018/8-151

Type:

SNAC Small

Principal Investigator:

Jason Serviss

Affiliation:

Karolinska Institutet

Start Date:

2018-05-01

End Date:

2019-05-01

Primary Classification:

30108: Cell and Molecular Biology

Webpage:

Allocation

Abstract

Advances in single-cell biology has enabled us to investigate isolated single cells at an unprecedented scale and resolution. However, cells in multicellular organisms are largely defined by their spatial organization within organ structures, which means that general methods for studying direct cell interaction on a large scale are needed. We propose a novel method, Cell Interaction by Multiplet Sequencing (CIM-Seq) that uses computational deconvolution of RNA-seq data from partially dissociated tissue to create cell interaction maps. CIM-Seq is a general method for cell interaction studies that can be used on cell types defined to an arbitrary resolution allowing identification of interacting sub-cell types or cell states.