SNIC
SUPR
SNIC SUPR
Lactobacillus reuteri R2LC
Dnr:

SNIC 2018/8-217

Type:

SNAC Small

Principal Investigator:

Hava Tomenius

Affiliation:

Uppsala universitet

Start Date:

2018-06-15

End Date:

2019-07-01

Primary Classification:

30109: Microbiology in the medical area

Webpage:

Allocation

Abstract

A lactobacillus reuteri strain, isolated from the gut of rats, was used to clone in a expression plasmid carrying a codon optimized human CXCL12 gene. The resulting strain has shown effectiveness in healing wounds when applied directly to the wound bed. This resulting, genetically modified organism, will be sequenced using PacBio technology. The resulting assembled genome and any plasmids will be used by NBIS bioinformatician to fine-tune the assembly with genome circularization. The NBIS bioinformatician will perform a full annotation of the core genome and plasmids using Prokka. Manual curation will be made possible through WebApollo. Future studies will be aimed at stability of the genome and expression plasmid through exposure to different conditions.